Journal
CANCER LETTERS
Volume 202, Issue 2, Pages 219-230Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2003.08.011
Keywords
phage display; cell targeting; lung cancer; peptides; peptide library
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Funding
- NCI NIH HHS [P50CA70907] Funding Source: Medline
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Discovery of ligands specific to receptor(s) on a surface of a cancer cell could impact clinical issues including functional diagnosis and cell-specific drug delivery. Using a phage display approach, we have isolated 20-mer peptide ligands that bind to 3 different human lung tumor cell lines, NCI-H1299, NCI-H2009, and A549. The panning protocol is unbiased with no selection pressure towards binding a particular cellular receptor. The isolated phage bind to their target cells 24-300 times better than a control phage. Furthermore, the isolated peptides display remarkable cell-specificities and are able to discriminate between normal and cancerous cells as well as different lung tumor cells. The cell-specificities are not coincident with tumor classes indicating that the peptides are able to recognize cell-surface features that are not represented within the classification of tumor type. The isolated peptides are functional outside of the context of the phage and multimerization of the peptide increases its affinity for its given cell type, thus expanding their utility in clinical situations. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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