Journal
FEBS LETTERS
Volume 556, Issue 1-3, Pages 211-215Publisher
WILEY
DOI: 10.1016/S0014-5793(03)01435-2
Keywords
phosphorylation; Ser/Thr kinase; focal adhesion
Funding
- NHLBI NIH HHS [HL52459] Funding Source: Medline
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Vasodilator-stimulated phosphoprotein (VASP), an actin binding protein localized to areas of focal contacts, is a substrate for the cyclic adenosine monophosphate/cyclic guanosine monophosphate (cAMP/cGMP)-dependent protein kinases (PKA, PKG). In this study, we show that serum stimulation of vascular smooth muscle cells (SMCs) induces VASP phosphorylation on Ser157, in a mechanism not dependent on PKA or PKG. We tested the possibility that protein kinase C (PKC), a regulator of cytoskeletal function, is involved. PKC inhibition or down-regulation prevented serum-induced phosphorylation of VASP at Ser157 in rat vascular SMCs. Additionally, recombinant PKCalpha directly phosphorylated Ser157 on VASP. In summary, our data support the hypothesis that PKC phosphorylates VASP and mediates serum-induced VASP regulation. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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