Journal
JOURNAL OF CELL BIOLOGY
Volume 164, Issue 1, Pages 89-96Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200310041
Keywords
apoptosis; cancer; Bcl-2; Apaf-1; caspase
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Funding
- NCI NIH HHS [P01 CA069381, R01 CA080188, CA 43540, CA 80188, CA 69381, R01 CA043540] Funding Source: Medline
- NIAID NIH HHS [AI 47891, R01 AI047891] Funding Source: Medline
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Based on experiments with cultured fibroblasts, the apoptosis regulators caspase-9 and Apaf-l are hypothesized to function as tumor suppressors. To investigate their in vivo role in lymphomagenesis, an IgH enhancer-driven c-myc transgene was crossed onto Apaf-1(-/-) and caspase-9(-/-) mice. Due to perinatal lethality, Emu-myc transgenic Apaf-1(-/-) or caspase-9(-/-) fetal liver cells were used to reconstitute lethally irradiated recipient mice. Surprisingly, no differences were seen in rate, incidence, or severity of lymphoma with loss of Apaf-l or caspase-9, and Apaf-l was not a critical determinant of anticancer drug sensitivity of c-myc-induced lymphomas. Moreover, loss of Apaf-l did not promote oncogene-induced transformation of mouse embryo fibroblasts. Thus, Apaf-l and caspase-9 do not suppress c-myc-induced lymphomagenesis and embryo fibroblast transformation.
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