Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 101, Issue 1, Pages 221-226Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2637010100
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Funding
- NCI NIH HHS [R01 CA086065, P20CA86312, CA86065, R24 CA092862, P01 CA049605, R24CA92862, R33CA88303, P01CA49605] Funding Source: Medline
- NIDDK NIH HHS [R01DK58664, R01 DK058664] Funding Source: Medline
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To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled hematopoietic stem cells (HSC) in irradiated recipients. Transplanted HSC generated discrete foci in the spleen and bone marrow (BM), at a frequency that correlated with BM compartment size. Initially detected foci could expand locally, seed other sites in BM or spleen, and/or recede with different kinetics. These studies reveal dynamic and variable patterns of engraftment from highly purified HSC and indicate that the final overall contribution of individual HSC to hematopoietic chimerism does not depend on the specific site of initial engraftment and expansion.
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