Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 5, Pages 1505-1513Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf505014n
Keywords
Moringa oleifera; isothiocyanates; 4-[(alpha-L-rhamnosyloxy)benzyl]isothiocyanate; 4-[(4 '-O-acetyl-alpha-L-rhamnosyloxy)]osptjopcuamate; polyphenols; NQO1; FCPC; antioxidants
Funding
- Botanical Research Center Pilot Program from the National Center for Complementary and Alternative Medicine (NCCAM) [5P50AT002776-08 S12-50318, P50AT002776-01]
- Office of Dietary Supplements (ODS)
- NIH Training Grant [T32:5T32AT004094-04]
- SENESCYT Fellowship from Ecuador
- Higher Education Council of Turkey
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Moringa oleifera Lam. is a fast-growing, tropical tree with various edible parts used as nutritious food and traditional medicine. This study describes an efficient preparatory strategy to extract and fractionate moringa leaves by fast centrifugal partition chromatography (FCPC) to produce polyphenol and isothiocyanate (ITC) rich fractions. Characterization and further purification of these fractions showed that moringa polyphenols were potent direct antioxidants assayed by oxygen radical absorbance capacity (ORAC), whereas moringa ITCs were effective indirect antioxidants assayed by induction of NAD(P)H quinone oxidoreductase 1 (NQO1) activity in Hepa1c1c7 cells. In addition, purified 4-[(a-l-rhamnosyloxy)benzyl]isothiocyanate and 4-[(4'-O-acetyl-a-l-rhamnosyloxy)benzyl]isothiocyanate were further evaluated for their ORAC and NQO1 inducer potency in comparison with sulforaphane (SF). Both ITCs were as potent as SF in inducing NQO1 activity. These findings suggest that moringa leaves contain a potent mixture of direct and indirect antioxidants that can explain its various health-promoting effects.
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