Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1023, Issue 1, Pages 159-163Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2003.09.022
Keywords
preparative chromatography; counter-current chromatography; Lithospermum erythrorhizon; plant materials; pharmaceutical analysis; shikonin
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The bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6 mg of shikonin at 98.9% purity was obtained from 52 mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200 min in a one-step separation. (C) 2003 Elsevier B.V. All rights reserved.
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