Journal
SCIENCE
Volume 303, Issue 5655, Pages 238-240Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1090934
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Funding
- NICHD NIH HHS [T32 HD-07516, HD-42026] Funding Source: Medline
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The imprinted regulation of H19 and Insulin-like growth factor 2 expression involves binding of the vertebrate insulator protein, CCCTC binding factor (CTCF), to the maternally hypomethylated differentially methylated domain (DMD). How this hypomethylated state is maintained during oogenesis and the role of CTCF, if any, in this process are not understood. With the use of a transgenic RNA interference (RNAi)-based approach to generate oocytes with reduced amounts of CTCF protein, we found increased methylation of the H19 DMD and decreased developmental competence of CTCF-deficient oocytes. Our results suggest that CTCF protects the H19 DMD from de novo methylation during oocyte growth and is required for normal preimplantation development.
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