Journal
FEBS LETTERS
Volume 557, Issue 1-3, Pages 209-214Publisher
WILEY
DOI: 10.1016/S0014-5793(03)01495-9
Keywords
PEST protein; ubiquitin-like domain; RING finger; ubiquitin ligase; cell cycle; proteasome
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We previously reported the association of a novel Np95/ICBP90-like RING finger protein (NIRF) with a novel PEST-containing nuclear protein (PCNP). NIRF is a nuclear protein with a ubiquitin-like domain, a PHD finger, a YDG/ SRA domain, Rb-binding motifs and a RING finger. In this study, we showed that NIRF has auto-ubiquitination activity, the hallmark of a ubiquitin ligase. PCNP was readily ubiquitinated in 293 and COS-7 cells, and NIRF ubiquitinated PCNP in vitro as well as in vivo. Considering that NIRF is implicated in cell cycle regulation, these findings suggest that NIRF and PCNP are a ubiquitin ligase and its substrate, respectively, and may constitute a novel signaling pathway with some relation to cell proliferation. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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