Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 16, Pages 4068-4079Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b00595
Keywords
polyphenols; fingerprints; ellagitannin; gallic acid; quinic acid; quercetin; kaempferol; myricetin; LC-MS/MS; MRM
Funding
- Turku University Foundation
- Academy of Finland [258992]
- University of Turku Graduate School (UTUGS)
- Kone Foundation
- EU Marie Curie Initial Training Network (LegumePlus) [PITN-GA-2011-289377]
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This paper,describes the development of a rapid method with ultraperformance liquid chromatography-triple-quadrupole mass spectrometry that can specifically measure group-specific fingerprints from plant extracts for the following polyphenol groups: (1) ellagitannins, (2) gallic acid derivatives, (3) quinic acid derivatives, (4) quercetin-based flavonol glycosides, (5) kaempferol-based flavonol glycosides, and (6) myricetin-based flavonol glycosides. In addition, the method records simultaneously diode array and full scan mass spectrometry data that can be used to later characterize and quantify the main individual polyphenols if necessary: All of this is achieved within the 10 min period of analysis, which makes the presented Method a significant addition to the,chemistry tools,currently available for the rapid,analysis of complex polyphenol mixtures from plant extracts.
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