Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 85, Issue 2, Pages 122-129Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.10865
Keywords
cell-free protein synthesis; urokinase; disulfide bond formation; protein folding; sulfhydrylredox potential
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In this report, we demonstrate that a complex mammalian protein containing multiple disulfide bonds is successfully expressed in an E.coli-based cell-free protein synthesis system. Initially, disulfide-reducing activities in the cell extract prevented the formation of disulfide bonds. However, a simple pretreatment of the cell extract with iodoacetamide abolished the reducing activity. This extract was still active for protein synthesis even under oxidizing conditions. The use of a glutathione redox buffer coupled with the DsbC disulfide isomerase and pH optimization produced 40 mug/mL of active urokinase protease in a simple batch reaction. This result not only demonstrates efficient production of complex proteins, it also emphasizes the control and flexibility offered by the cell-free approach. (C) 2004 Wiley Periodicals, Inc.
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