Journal
EMBO JOURNAL
Volume 23, Issue 2, Pages 354-364Publisher
WILEY
DOI: 10.1038/sj.emboj.7600053
Keywords
polyadenylation; RNA polymerase IICTD; termination; transcription
Categories
Funding
- NIGMS NIH HHS [GM56663, GM46498, R01 GM056663, R01 GM046498] Funding Source: Medline
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To understand the factor interactions of transcribing RNA polymerase II (RNApII) in vivo, chromatin immunoprecipitations were used to map the crosslinking patterns of multiple elongation and polyadenylation factors across transcribed genes. Transcription through the polyadenylation site leads to a reduction in the levels of the Ctk1 kinase and its associated phosphorylation of the RNApII C-terminal domain. One group of elongation factors (Spt4/5, Spt6/Iws1, and Spt16/Pob3), thought to mediate transcription through chromatin, shows patterns matching that of RNApII. In contrast, the Paf and TREX/THO complexes partially overlap RNApII, but do not crosslink to transcribed regions downstream of polyadenylation sites. In a complementary pattern, polyadenylation factors crosslink strongly at the 30 ends of genes. Mutation of the 30 polyadenylation sequences or the Rna14 protein causes loss of polyadenylation factor crosslinking and readthrough of termination sequences. Therefore, transcription termination and polyadenylation involve transitions at the 30 end of genes that may include an exchange of elongation and polyadenylation/ termination factors.
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