Temperature-dependent Raman spectra of collagen and DNA

Raman spectra of collagen and DNA were discussed at different temperatures. The temperature-dependence of Raman intensity was obtained in the region from -150 to 200 degreesC. Four denaturation points at 0, 40, 68 and 90degreesC of collagen and two peaks at 38 and 82 degreesC for DNA were obtained. The wavenumbers of many vibrational modes were found to increase for lower temperature, but the peak at 1302 cm(-1) of collagen and the peak at 1101 cm(-1) of DNA showed the opposite trend. In all of the vibrational modes of DNA, the bases showed the most sensitive to different temperatures and there is a pronounced shift of bands at 70degreesC, the starting point of denaturation. (C) 2003 Elsevier B.V. All tights reserved.