Journal
HYPERTENSION
Volume 43, Issue 2, Pages 312-316Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/01.HYP.0000111835.16662.43
Keywords
renin-angiotensin system; sympathetic nervous system; hypertension, genetic; receptors, angiotensin II; losartan; mice
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Funding
- NHLBI NIH HHS [P01 HL14388] Funding Source: Medline
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In addition to central nervous system actions, angiotensin (Ang) II may increase sympathetic nerve activity (SNA) via a direct action on sympathetic ganglia. We hypothesized that sympathetic ganglionic actions of endogenous Ang II contribute to SNA in transgenic mice that overexpress renin and angiotensinogen (R(+)A(+) mice). Renal SNA and arterial pressure were recorded in anesthetized R(+)A(+) and littermate control mice before and after ganglionic blockade, and after additional blockade of angiotensin type 1 (AT(1)) receptors with losartan. Ganglionic blockade essentially abolished SNA in control mice, but only reduced SNA to 47+/-18% of baseline in R(+)A(+) mice. The residual SNA remaining after ganglionic blockade in R(+)A(+) mice was reduced from 47+/-18% to 8+/-6% of baseline by losartan (P<0.05). The sympathoinhibitory response to losartan was accompanied by an enhanced decrease in arterial pressure in R(+)A(+) mice compared with that observed in control mice. AT(1) receptor expression in sympathetic ganglia, as measured by real-time reverse transcription-polymerase chain reaction, was increased approximate to 3-fold in R(+)A+ versus control mice. The results demonstrate that, as anticipated, essentially all of the renal postganglionic SNA in control mice is driven by preganglionic input. The major new finding is that Ang II-evoked ganglionic activity accounts for approximate to 40% of total SNA in R(+)A(+) mice. The significant contribution of the direct ganglionic action of Ang II in R(+)A(+) mice likely reflects both increased levels of Ang II and upregulation of AT(1) receptors in sympathetic ganglia.
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