Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 10, Pages 2734-2740Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b00028
Keywords
Ganoderma atrum polysaccharide; spleen lymphocytes; immunomodulatory; effect; signaling pathway; interleukin-2
Funding
- National Key Technology Research and Development Program of China [2012BAD33B06]
- National Natural Science Foundation of China [31130041]
- Program for New Century Excellent Talents in University [NCET-12-0749]
- Research Program of State Key Laboratory of Food Science and Technology [SKLF-ZZA-201301]
- Project of Science and Technology of Jiangxi Provincial Education Department [KJLD13004]
- Key Project of International Cooperation of Jiangxi Provincial Department of Science and Technology [20141BDH80009]
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The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of Ganoderma atrum polysaccharide (PSG-1) in spleen lymphocytes. Our results showed that PSG-1 increased the intracellular Ca2+ concentration and calcineurin (CaN) activity. Moreover, PSG-1 was found to elevate nuclear factor of activated T cells (NFAT) activity, but this effect could be diminished by the treatment of CaN inhibitors (cyclosporin A and FK506). PSG-1-induced interleukin (IL)-2 production was also inhibited by cyclosporin A and FK506. In addition, PSG-1 was found to significantly enhance protein kinase C (PKC) activity. PKC was involved in induction of NFAT activity by PSG-1, as evidenced by abrogation of NFAT activity by PKC inhibitor calphostin C, which significantly decreased PSG-1-induced IL-2 production. On the basis of these results, we concluded that PSG-1 may induce activation of spleen lymphocytes at least in part via the Ca2+/CaN/NFAT/IL-2 signaling pathway and the PKC/NFAT/IL-2 signaling pathway cooperatively regulated PSG-1-induced activation of spleen lymphocytes.
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