Lipid and protein oxidation in vitro, and antioxidant potential in meat from Charolais cows finished on pasture or mixed diet

In this work, the effect of finishing diet (pasture- or mixed-diet) on lipid and protein oxidation in beef homogenates was evaluated. Oxidation was chemically induced by ferrous iron and hydrogen peroxide. Lipid and protein oxidation were respectively measured by determining TBA reactive substances (TBA-RS) and protein carbonyl groups (DNPH coupling method). Evaluation of the antioxidant status of meat was also performed by measuring vitamin E concentration and antioxidant enzyme activities: superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase. Pasture-finishing mode of animals significantly protected lipids in meat from oxidation but diet mode did not affect protein oxidation. Concentration of vitamin E was higher in meat from pasture-fed animals. Different diets had opposite effects on SOD and GPx activities, pasture-finishing mode of animals increasing SOD activity but decreasing GPx activity. No significant effect of diet was noted on catalase activity. (C) 2003 Elsevier Ltd. All rights reserved.