Journal
PLANT CELL REPORTS
Volume 22, Issue 7, Pages 509-512Publisher
SPRINGER
DOI: 10.1007/s00299-003-0698-2
Keywords
acetolactate synthase; chimeric RNA/DNA oligonucleotide; gene targeting; herbicide resistance; Oryza sativa L.
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Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1x10(-4). These results demonstrate that CO-directed gene targeting is feasible in rice.
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