Semi-automated 86Y purification using a three-column system

The separation of Y-86 from Sr-86 was optimized by a semi-automated purification system involving the passage of the target sample through three sequential columns. The target material was dissolved in 4 N HNO3 and loaded onto a Sr-selective (Sr-Spec) column to retain the Sr-86. The yttrium was eluted with 4 N HNO3 onto the second Y-selective (RE-Spec) column with quantitative retention. The RE-Spec column was eluted with a stepwise decreasing concentration of HNO3 to wash out potential metallic impurities to a waste container. The eluate was then pumped onto an Aminex A5 column with 0.1 N HCl and finally with 3 N HCl to collect the radioyttrium in 0.6-0.8 mL with a >80% recovery. This method enabled us to decontaminate Sr by 250,000 times and label 30 mug of DOTA-Biotin with a >95% yield. (C) 2004 Elsevier Inc. All rights reserved.