Journal
BIOCHEMISTRY-MOSCOW
Volume 69, Issue 2, Pages 216-219Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1023/B:BIRY.0000018954.38006.9a
Keywords
EUPERGIT(R) C-250L; carbonic anhydrase; affinity chromatography
Categories
Ask authors/readers for more resources
A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGIT(R) C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ionic strengths. Human carbonic anhydrase isozymes (HCA I and HCA II) and bovine carbonic anhydrase (BCA) were purified in high yields from erythrocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available