Embryonic chromosomal abnormalities obtained after rescue intracytoplasmic sperm injection of 1-day-old unfertilized oocytes

Purpose: To study if second day intracytoplasmic sperm injection (ICSI) results in chromosomal abnormalities in the embryos. Methods: Rescue ICSI was performed on 14 metaphase II (MII) oocytes after unsuccessful conventional IVF, four were fertilized. Fluorescent in situ hybridization ( FISH) was performed on these four embryos and was informative for three. Results: There were two tetraploid embryos, one mosaic embryo with trisomy 21, tetrasomy 18, and tetrasomy for sex chromosomes in one cell and trisomy 22 in another cell. Conclusions: We discourage the use of second day ICSI due to the observed increase in chromosomal abnormalities in these embryos.