Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 378, Issue 3, Pages 655-663Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-003-2217-2
Keywords
steroid hormones; reporter gene; endocrine disrupters; residues
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We have studied stable transformed human mammary cell lines with highly inducible steroid receptor-mediated luciferase reporter gene expression. Cells responding specifically to glucocorticoids, progestagens, androgens, or estrogens are described and characterized. The use of this high-throughput, cell-based assay for analysis of steroid (ant)agonists is reported. Systematic characterization of endocrine-disrupting activity on human receptors and in a human-cell system is interpreted for a selection of xenobiotics. We show that the phytoestrogens apigenin and genistin have progestagenic and androgenic activity, respectively. Finally, application of cell-based assays to the analysis of environmental samples is discussed.
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