Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 1, Pages 78-84Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b04747
Keywords
Bacillus amyloliquefaciens K11; keratinase; extreme alkaline; gene expression
Funding
- Twelfth Five-Year National Science and Technology Project in Rural Areas of China [2013BAD10B01-2]
- National High Technology Research and Development Program of China (863 program) [2013AA102803]
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Keratinase is essential to degrade the main feather component, keratin, and is of importance for wide industrial applications. In this study, Bacillus amyloliquefaciens strain K11 was found to have significant feather-degrading capacity (completely degraded whole feathers within 24 h). The keratinase encoding gene, kerK, was expressed in Bacillus subtilis SCK6. The purified recombinant KerK showed optimal activity at 50 degrees C and pH 11.0 and degraded whole feathers within 0.5 h in the presence of DTT. The recombinant plasmids harboring kerK were extracted from B. subtilis SCK6 and transformed into B. amyloliquefaciens K11. As a result, the recombinant B. amyloliquefaciens K11 exhibited enhanced feather-degrading capacity with shortened reaction time within 12 h and increased keratinolytic activity (1500 U/mL) by 6-fold. This efficient and rapid feather-degrading character makes the recombinant strain of B. amyloliquefaciens K11 have potential for applications in feather meal preparation and waste feather disposal.
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