Journal
PROTEIN JOURNAL
Volume 23, Issue 2, Pages 135-141Publisher
KLUWER ACADEMIC/PLENUM PUBL
DOI: 10.1023/B:JOPC.0000020080.21417.ff
Keywords
diphenolase; dodecylresorcinol; hexylresorcinol; inhibition; monophenolase; mushroom tyrosinase
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The effects of hexylresorcinol and dodecylresorcinol on the monophenolase and diphenolase activity of mushroom tyrosinase have been studied. The results show that hexylresorcinol and dodecylresorcinol can inhibit both monophenolase and diphenolase activity of the enzyme. The lag period of the enzyme was obviously lengthened, and the steady-state activity of the enzyme decreased sharply. Two muM of hexylresorcinol and dodecylresorcinol can lengthen the lag period from 98 s to 260 and 275 s, respectively. Both hexylresorcinol and dodecylresorcinol can lead to reversible inhibition of the enzyme. The IC50 values of hexylresorcinol and dodecylresorcinol were estimated as 1.24 and 1.15 muM for monophenolase and as 0.85 and 0.80 muM for diphenolase, respectively. A kinetic analysis shows that hexylresorcinol and dodecylresorcinol are competitive inhibitors. The apparent inhibition constant for hexylresorcinol and dodecylresorcinol binding with free enzyme has been determined to be 0.443 and 0.405 muM for diphenolase, respectively.
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