Poly(amidoamine) dendrimers as nanoscale diffusion probes in sol-gel films investigated by total internal reflection fluorescence spectroscopy

Three generations of poly(amidoamine) dendrimers were dye-labeled and chemically modified to have terminal carboxyl groups and used as variably sized probes to study diffusion in thin sol-gel films. Total internal reflection fluorescence spectroscopy experiments, both correlation and concentration-jump measurements, were employed to measure the relative populations and effective diffusion coefficients of dendrimers in the films. For films prepared from small (27-nm) silica particles, larger dendrimers could be completely excluded from penetrating the sol-gel structure. In films made of larger (150-nm) particles with correspondingly larger pores, concentration-jump experiments showed that larger dendrimers are excluded from more of the intraparticle pore space than small dendrimers. Similarly, fluorescence-correlation measurements showed that the diffusion of smaller dendrimers exhibited greater tortuosity than larger dendrimers in the interparticle pores of the film. The smaller dendrimers explore a greater volume of smaller, more convoluted pores, whereas larger dendrimers penetrate a smaller volume of larger, more open pores.