Journal
ANALYTICAL BIOCHEMISTRY
Volume 325, Issue 2, Pages 293-300Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2003.11.005
Keywords
DNA; human genome; atomic force microscopy/microscope; evaporating liquid drops; developed fluid capillary flow method; mica
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A circular bacterial artificial chromosome of 148.9 kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of +/-3.6%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM. (C) 2003 Elsevier Inc. All rights reserved.
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