Evidence of caspase-3 activation in hyposmotic stress-induced necrosis

Primary culture of dentate gyrus was submitted to a hyposmotic stress that induces a rapid cell death that is necrosis morphologically. Surprisingly, we observed a rapid and dramatic upregulation of the active form of caspase-3 (caspase-3(a)) in both neurons and glial cells. Caspase-3(a) immunoreactivity appears as early as 1 min after hyposmotic treatment, when some neurons are still alive, suggesting that caspase-3a may contribute to further necrotic cell death. (C) 2003 Elsevier Ireland Ltd. All rights reserved.