Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1027, Issue 1-2, Pages 231-235Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)01300-1
Keywords
detection; electrophoresis; chip technology; instrumentation; calcium fluoride; amino acids
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In this paper, we demonstrate microfluidic capillary electrophoresis (CE) devices made in CaF2, for optical detection in a broad spectral range. We have desiged methods for micromachining and enclosing capillaries in CaF2. The utility of these microdevices has been shown through CE analysis of fluorescently labeled amino acids. We have also performed infrared spectroscopy for analyte identification in microfluidic CaF2 channels. These CaF2 microdevices open the door to microchip separations with optical detection in the ultraviolet, visible, and infrared spectral regions. (C) 2003 Elsevier B.V. All rights reserved.
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