Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 42, Pages 9277-9285Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b04141
Keywords
lotus leaves; sedative-hypnotic; anxiolytic; GABA(A) receptor; monoaminergic system
Funding
- International Scientific and Technological Cooperation from Ministry of Science and Technology of the People's Republic of China [1108, 2011DFA32730]
- National Mega-project for Innovative Drugs from Ministry of Science and Technology of the People's Republic of China [2012ZX09301002-001]
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Lotus leaves have been used traditionally as both food and herbal medicine in Asia. Open-field, sodium pentobarbital-induced sleeping and light/dark box tests were used to evaluate sedative-hypnotic and anxiolytic effects of the total alkaloids (TA) extracted from the herb, and the neurotransmitter levels in the brain were determined by ultrafast liquid chromatography-tandem mass spectrometry. The effects of picrotoxin, flumazenil, and bicuculline on the hypnotic activity of TA, as well as the influence of TA on Cl- influx in cerebellar granule cells, were also investigated. TA showed a sedative hypnotic effect by increasing the brain level of gamma-aminobutyric acid (GABA), and the hypnotic effect could be blocked by picrotoxin and bicuculline, but could not be antagonized by flumazenil. Additionally, TA could increase Cl- influx in cerebellar granule cells. TA at 20 mg/kg induced anxiolytic-like effects and significantly increased the concentrations of serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIA(A)), and dopamine (DA). These data demonstrated that TA exerts sedative-hypnotic and anxiolytic effects via binding to the GABA(A) receptor and activating the monoaminergic system.
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