Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1028, Issue 1, Pages 175-177Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2003.11.087
Keywords
sulfamonomethoxine; sulfadimetboxine
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A hazardous-chemical free method for simultaneous determination of sulfamonomethoxine (SMM), sulfadimethoxine (SDM), and their N-4-acetyl metabolites in raw milk using shielded column liquid chromatography is developed. The target analytes are extracted by mixing with ethanol-acetic acid (97:3, v/v) followed by centrifugation. The procedure uses a Hisep shielded hydrophobic phase (SUP) column, isocratic elution with 0.1% acetic acid solution (pH 3.1, in water)-ethanol (75:25, v/v), and a photo-diode array detector. Average recoveries from samples spiked at 25-500 ng/ml for each drug were >81% with relative standard deviations within 5%. The limits of quantitation were <25 ng/ml. (C) 2004 Elsevier B.V. All rights reserved.
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