Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 37, Pages 8199-8208Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b03833
Keywords
alpha,omega-dicarboxylic acids; CYP450s; monooxygenase; omega-oxidation pathway; fatty acids
Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning (MSIP) [NRF-2009-C1AAA001-2009-0093491]
- Next-Generation Bio Green 21 Program (SSAC) [PJ011181]
- Rural Development Administration, Republic of Korea
- National Research Foundation of Korea [22A20130012653, 2009-0093491] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Long-chain alpha,omega-dicarboxylic acids (LDCAs, >= C12) are widely used as a raw material for preparing various commodities and polymers. In this study, a CYP450-monooxygenase-mediated omega-oxidation pathway system with high omega-regioselectivity was heterologously expressed in Escherichia coli to produce DCAs from fatty acids. The resulting engineered E. coli produced a maximum of 41 mg/L of C12 DCA and 163 mg/L of C14 DCA from fatty acids (1 g/L), following 20 h of whole cell biotransformation. Addition of a heme precursor and the hydroxyl radical scavenger, thiourea, increased product concentration (159 mg/L of C12 DCA and 410 mg/L of C14 DCA) in a shorter culture duration than that of the corresponding controls. DCAs of various chain lengths were synthesized from coconut oil hydrolysate using the engineered E. coli. This novel synthetic biocatalytic system could be applied to produce high value DCAs in a cost-effective manner from renewable plant oils.
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