4.1 Article

Varietal identification of coffee seeds by RAPD technique

Journal

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
Volume 47, Issue 1, Pages 7-11

Publisher

INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132004000100002

Keywords

Coffea; cultivar identification; molecular marker

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This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained,from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.

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