Journal
BIOMACROMOLECULES
Volume 5, Issue 2, Pages 537-546Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm034387k
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Guar gum, a beta-D-(1-4)-linked D-mannan with alpha-D-galactopyranosyl units attached as side groups, was treated with a-galactosidase, an enzyme that splits off the alpha-D-galactosyl units to obtain a galactomannan with a low galactose content. The galactose-depleted polysaccharide was then selectively oxidized in C(6) position and epimerized using mannuronan C(5)-epimerases, namely AlgE1, AlgE4, AlgE6, and their mixtures, obtaining new pseudo-alginates. In this paper, we report a full high field ID and 2D NMR study of guar gum as such and of the galactose-depleted, oxidized and epimerized compounds, respectively. From the H-1 NMR spectra, the degree of epimerization, the distribution of mannuronic acid (M) and guluronic acid (G) residues and the average G-block length, N-G>1, were obtained. By means of NMR diffusion experiments, it was also shown that no significant degradation of the polysaccharide occurs as a consequence of the epimerization reactions.
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