Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 113, Issue -, Pages 173-180Publisher
HUMANA PRESS INC
DOI: 10.1385/ABAB:113:1-3:173
Keywords
solid-state fermentation; lipase; Penicillium; soy cake; olive oil; moisture
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The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.
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