Journal
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
Volume 36, Issue 3, Pages 405-410Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2003.12.005
Keywords
inflammation; endothelium; chemokine; interleukin; C-reactive protein
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Funding
- NCCIH NIH HHS [K24 AT00596] Funding Source: Medline
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Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRR Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31 %, P < 0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity. (C) 2003 Elsevier Ltd. All rights reserved.
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