Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 56, Issue 3, Pages 427-430Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2003.11.020
Keywords
cat; cesium chloride; oocyst; purification; Toxoplasma gondii
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We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a >96% recovery of very pure unsporulated or sporulated oocysts, but requires fresh oocysts (: 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T gondii oocysts. (C) 2004 Elsevier B.V. All rights reserved.
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