Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 22, Issue 2, Pages 89-96Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420410001692769
Keywords
alcohol dehydrogenase; glucose dehydrogenase; ionic liquid; organic solvent; oxidoreductase; two phase system
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This review discusses recent achievements in the field of cofactor regeneration for the nicotinamide cofactors NADH and NADPH. The examples discussed include alcohol dehydrogenases, formate dehydrogenase, glucose dehydrogenase and a hydrogenase. For the reaction either one-phase systems or two-phase systems in combination with an organic solvent are discussed. For the enantioselective reduction of 2-octanone to ( R )-2-octanol it could be shown that enzyme coupled NADPH regeneration with glucose dehydrogenase and glucose results in shorter reaction times and higher yields when compared to the substrate coupled regeneration with 2-propanol.
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