Novel Soluble Dietary Fiber-Tannin Self-Assembled Film: A Promising Protein Protective Material

In this experiment, a natural promising protein protective film was fabricated through soluble dietary fiber (SDF)-tannin nanoduster self-assembly. FT-IR, XRD, and DSC tests were employed to investigate the interaction between the SDF and tannins before and after cross-linking induced by calcium ion. On the other hand, referring to the SEM and TEM results, the self-assembly process of the protein protective film could be indicated as follows: first, calcium ion, with its crossability, served as the nucleus; SDF and tannins were combined to prepare the nanoscale SDF tannin clusters; then, the dusters were homogeneously deposited on the surface of protein to form a protective film by self-assembling hydrogen bond between tannin component of clusters as adhesive and protein in aqueous solutions under very mild conditions. Film thickness could also be controlled by tannin of different concentrations tanging from 114 to 1384 mu m. Antibacterial test and in vitro cytotoxicity test proved that the film had a broad spectrum of antimicrobial properties and excellent cell hiocompatibility, respectively, which might open up new applications in the food preservation and biomedical fields.