Journal
TISSUE ENGINEERING
Volume 10, Issue 3-4, Pages 411-420Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/107632704323061771
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Funding
- NCRR NIH HHS [P41-RR01861] Funding Source: Medline
- NHLBI NIH HHS [R01 HL067377, HL67377] Funding Source: Medline
- NIAMS NIH HHS [AR47637-01A1, R01 AR047637] Funding Source: Medline
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The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.
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