Journal
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volume 447, Issue 6, Pages 840-844Publisher
SPRINGER
DOI: 10.1007/s00424-003-1214-9
Keywords
creatine kinase; hexokinase; transgenic mouse; VDAC; diffusion
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The response of mitochondrial oxygen consumption to ADP in saponin-skinned cardiac fibre bundles has an apparent K-m an order of magnitude higher than that in isolated mitochondria. Here we report that incubating skinned cardiac fibre bundles from wild-type mice or double-knockout mice lacking both cytosolic and mitochondrial creatine kinase (CK) with CK and creatine or with yeast hexokinase and glucose as extramitochondrial ADP-producing systems decreases the apparent K-m of the bundles for ADP severalfold. We conclude that the affinity of mitochondria for ADP in mouse heart is of the same order of magnitude as that of isolated mitochondria, while the high apparent K-m of the bundles is caused by diffusion gradients outside the mitochondria.
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