Journal
JOURNAL OF CELL SCIENCE
Volume 117, Issue 7, Pages 1117-1128Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.00939
Keywords
Adenomatous polyposis coli; EB1; centrosome; mother centriole; microtubule
Categories
Funding
- NIGMS NIH HHS [GM52022, R01 GM052022, T32GM07276-27, T32 GM007276, GM07365, T32 GM007365, R01 GM078270] Funding Source: Medline
- NINDS NIH HHS [R01 NS042735, NS42735] Funding Source: Medline
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Adenomatous polyposis coli (APC) and End-binding protein 1 (EB1) localize to centrosomes independently of cytoplasmic microtubules (MTs) and purify with centrosomes from mammalian cell lines. Localization of EB1 to centrosomes is independent of its MT binding domain and is mediated by its C-terminus. Both APC and EB1 preferentially localize to the mother centriole and EB1 forms a cap at the end of the mother centriole that contains the subdistal appendages as defined by epsilon-tubulin localization. Like endogenous APC and EB1, fluorescent protein fusions of APC and EB1 localize preferentially to the mother centriole. Depletion of EB1 by RNA interference reduces MT minus-end anchoring at centrosomes and delays MT regrowth from centrosomes. In summary, our data indicate that APC and EB1 are functional components of mammalian centrosomes and that EB1 is important for anchoring cytoplasmic MT minus ends to the subdistal. appendages of the mother centriole.
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