Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 112, Issue 3, Pages 163-172Publisher
HUMANA PRESS INC
DOI: 10.1385/ABAB:112:3:163
Keywords
biosurfactant; Bacillus subtilis; cassava effluent; surfactin; protease
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A cassava flour-processing effluent (manipueira) was evaluated as a substrate for surfactant production by two Bacillus subtilis strains. B. subtilis ATCC 21332 reduced the surface tension of the medium to 25.9 mN/m, producing a crude biosurfactant concentration of 2.2 g/L. The wild-type strain, B. subtilis LB5a, reduced the surface tension of the medium to 26.6 mN/m, giving a crude biosurfactant concentration of 3.0 g/L. A decrease in surfactant concentration observed for B. subtilis ATCC 21332 seemed to be related to an increase in protease activity. The biosurfactant produced on cassava effluent medium by B. subtilis LB5a was similar to surfactin.
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