Journal
BIOMEDICAL MICRODEVICES
Volume 6, Issue 1, Pages 75-80Publisher
SPRINGER
DOI: 10.1023/B:BMMD.0000013369.34716.8d
Keywords
polymerase chain reaction; lab on a chip; microfluidics; microfluidic platform; materials compatibility
Funding
- NCPDCID CDC HHS [NCI R01-CA78848-04] Funding Source: Medline
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We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.
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