Journal
JOURNAL OF BACTERIOLOGY
Volume 186, Issue 6, Pages 1678-1682Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.186.6.1678-1682.2004
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Variations in genome size and gene order were observed in archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR analysis of the same strain revealed a genomic duplication that underwent a translocation. Other strains had smaller duplications and deletions. These results demonstrate that storage in stabs over time at room temperature not only allows for further bacterial growth but also may produce an environment that selects for a variety of mutations, including genomic rearrangements.
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