Journal
FEMS YEAST RESEARCH
Volume 4, Issue 6, Pages 649-654Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsyr.2003.12.009
Keywords
Schizosaccharomyces pombe; alcohol dehydrogenase; adh1(+); SPAC5H10.06C; ethanol fermentation
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In the fission yeast Schizosaccharomyces pombe, only one alcohol dehydrogenase gene, adh1(+), has been identified. To elucidate the influence of adh1(+) on ethanol fermentation, we constructed the adh1 null strain (Deltaadh1). The Deltaadh1 cells still produced ethanol and grew fermentatively as the wild-type cells. Both DNA microarray and RT-PCR analysis demonstrated that this ethanol production is caused by the enhanced expression of a Saccharomyces cerevisiae ADH4-like gene product (SPAC5H10.06C named adh4(+)). Since the strain lacking both adh1 and adh4 genes (Deltaadh1 Deltaadh4) showed non-fermentative retarded growth, only these two ADHs produce ethanol for fermentative growth. This is the first observation that a S. cerevisiae ADH4-like alcohol dehydrogenase functions in yeast ethanol fermentation. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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