Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 68, Issue 3, Pages 609-614Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.68.609
Keywords
Clostridium stercorarium; beta-xylosidase
Ask authors/readers for more resources
The Clostridium stercorarium F-9 xyl43B gene encoding the beta-xylosidase Xyl43B consists of an open reading frame of 1,491 nucleotides that encodes a putative protein, classified in family 43, of 497 amino acids with a predicted molecular weight of 56,355. The deduced amino acid sequence of Xyl43B has sequence similarity with beta-xylosidases from Bacteriodes thetaiotaomicron (57% sequence identity), Prevotella ruminicola (45%), Streptomyces coelicolor (40%), and Clostridium acetobutylicum (36%), all of which have been classified in family 43 of the glycoside hydrolases. Xyl43B was purified from a recombinant Escherichia coli and characterized. The optimum pH of the purified enzyme was 3.5 and it was stable over pH from 3.0 to 8.0. Its optimum temperature was 80degreesC and it showed thermostability in the temperature range from 50 to 70degreesC. Xyl43B had a K of 6.2 mM and a V-max of 15 mumol min(-1) mg(-1) for p-nitrophenyl-beta-D-xylopyranoside.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available