Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 56, Issue 3, Pages 375-382Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2003.11.008
Keywords
plate assays; chromogenic substrates; polysaccharide-degrading bacteria; xylan-cibacron brilliant red 3B-A; ainylose-cibacron brilliant red 3B-A; HE-cellose-reactive orange 14
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A plate assay based on the visible solubilization of small Substrate particles and the formation of haloes oil Petri dishes, containing a mixture of different dye-labelled polysaccharides Lis substrates, provides a specific, reliable and rapid simultaneous detection of corresponding polysaccharide-degrading microorganisms. It has potential for increasing the efficacy of screening of microorganisms, utilizing different polysaccharides, in large numbers of natural samples. Diversely colored insoluble forms of amylose, xylan and hydroxyethyl-cellulose (HE-cellulose) were prepared as chromogenic substrates by using the cross-linking reagent 1,4-butanediol diglycidyl ether and the dyes Brilliant Red 3B-A, Cibacron Blue 3GA and Reactive Orange 14. Using the method, the bacteria with amylase or xylanase or cellulase or a combination of these activities were screened from soil and sludge samples, selected and identified according to 16S rDNA sequencing. (C) 2003 Elsevicr B.V. All Lights reserved.
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