MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAATβ

Lysophosphatidic Acid Acyltransferase beta (LPAAT beta) may be critically involved in osteosarcoma cell proliferation. However, the comprehensive mechanisms responsible for regulation of LPAAT beta in osteosarcoma cells remain unclear. This study found that enhanced LPAAT beta expression was correlated with osteosarcoma cell proliferation. MiR-24, targeted to LPAAT beta, was down-regulated in osteosarcoma cells. Overexpression of miR-24 down-regulated LPAAT beta expression in osteosarcoma cells. Specifically, overexpression of miR-24 inhibited osteosarcoma cell proliferation, however, such effect was blocked when LPAAT beta activity was inhibited. In conclusion, our study indicates that miR-24 is reduced in osteosarcoma cells, contributing to up-regulation of LPAAT beta and resultant osteosarcoma cell proliferation. (C) 2013 Elsevier Inc. All rights reserved.