Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 512, Issue 1, Pages 24-29Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2011.04.020
Keywords
Ethanol; Exercise; Lactate; Pyruvate; Rat; Drosophila
Categories
Funding
- Spanish Ministry of Education and Science (MEC) [SAF2008-00270, SAF2009-08334, SAF2010-19498]
- Conselleria de Educacion de la Generalitat Valenciana [PROMETEO/2010/074]
- Red Tematica de investigacion cooperativa en envejecimiento y fragilidad (RETICEF) [ISCIII2006-RED13-027]
- EU [COSTB35]
- Spanish Ministry of Innovation and Science [DPS2008-06968]
- FEDER from the European Union
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Sirtuins are deacetylases involved in metabolic regulation and longevity. Our aim was to test the hypothesis that they are subjected to redox regulation by the [NADH]/[NAD(+)] ratio. We used NIH3T3 fibroblasts in culture, Drosophila fed with or without ethanol and exercising rats. In all three models an increase in [NADH]/[NAD(+)] came up with an increased expression of sirtuin mRNA and protein. PGC-1 alpha (a substrate of sirtuins) protein level was significantly increased in fibroblasts incubated with lactate and pyruvate but this effect was lost in fibroblasts obtained from sirtuin-deficient mice. We conclude that the expression of sirtuins is subject to tight redox regulation by the [NADH]/[NAD(+)] ratio, which is a major sensor for metabolite availability conserved from invertebrates to vertebrates. (c) 2011 Published by Elsevier Inc.
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