Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 81, Issue 3, Pages 281-284Publisher
AMER OIL CHEMISTS SOC A O C S PRESS
DOI: 10.1007/s11746-004-0896-5
Keywords
Esterification; lipase; organic solvent
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The enzymatic synthesis of glycerides from glycerol and oleic acid in organic solvent was studied, and the optimal conditions for glyceride synthesis by lipases were established. Of the commercially available lipases that were investigated, Candida rugosa lipase and porcine pancreas lipase resulted in the highest extent of esterification. Iso-octane and hexane were particularly useful organic solvents in glyceride synthesis. The water content in the reaction mixture was of primary importance. For C. rugosa lipase and porcine pancreas lipase, the optimal water contents were 5 and 1%, respectively. Candida rugosa lipase and porcine pancreas lipase manifested contrasting positional specificities in glyceride synthesis.
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