Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 496, Issue 1, Pages 38-44Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2010.01.012
Keywords
Ascorbic acid; Dehydroascorbic acid; Glucose transporter; Senescence marker protein-30; Sodium-dependent vitamin C transporter
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Funding
- Ministry of Education, Science, and Culture, Japan
- Smoking Research Foundation, Japan
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In this study, we examined whether ascorbic acid (AA) and dehydroascorbic acid (DHA), the oxidized form of AA, levels in tissues regulate the AA transporters, sodium-dependent vitamin C transporters (SVCT) 1 and SVCT2 and DHA transporters, glucose transporter (GLUT) 1, GLUT3, GLUT4 mRNA by using senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice. These mice are incapable of synthesizing AA in vivo. AA depletion enhanced SVCT1 and SVCT2 mRNA expression in the liver and SVCT1 and GLUT4 mRNA expression in the small intestine, but not in the cerebrum or kidney. Next, we examined the actual impact of AA uptake by using primary cultured hepatocytes from SMP30/GNL KO mice. In the AA-depleted hepatocytes from SMP30/GNL KO mice, AA uptake was significantly greater than in matched cultures from wild-type mice. These results strongly affirm that intracellular AA is an important regulator of SVCT1 and SVCT2 expression in the liver. (C) 2010 Elsevier Inc. All rights reserved.
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