Determination of methimazole and carbimazole by flow-injection with chemiluminescence detection based on the inhibition of the Cu(II)-catalysed luminol-hydrogen peroxide reaction

This paper reports an indirect flow-injection (FI) method for the determination of the anti-hyperthyroid drugs methimazole and carbimazole in pharmaceuticals. The method was based on the inhibition that these thioimidazole drugs caused on the Cu(II)-catalysed chemiluminescence (CL) reaction between luminol and H2O2. The CL reaction was induced on-line and injection of the sample produced negative peaks as a result of the Cu(II) complexation by the analytes. The height of the FI peaks was proportional to the drug concentration in the sample. The linear range was 2-100 and 3-120 mg l(-1) for methimazole and carbimazole, respectively. The relative standard deviation was 1.9% for methimazole and 2.1% for carbimazole at the 50 mg l(-1) level (n = 10). Common excipients present in pharmaceutical tablets were found not to interfere with the analysis. The method was applied to the determination of methimazole and carbimazole in pharmaceutical formulations with recoveries in the range 100 +/- 4%. (C) 2003 Elsevier Science B.V. All rights reserved.